Serum Choline Plasmalogen is a Reliable Biomarker for Atherogenic Status
نویسندگان
چکیده
Plasmalogens (Pls) belong to a subclass of glycerophospholipids, and widely distributed in human and animal tissues (Nagan & Zoeller, 2001). Although the clinical significance of these phospholipids is recognized in relation to peroxisomal disorders (Wanders & Waterham, 2006), the physiopathological roles of Pls are not fully understood. Recently, serum (or plasma) Pls have gained interest in several clinical symptoms of life-style related disease possibly because of their antioxidant properties (Brites et al., 2004). We have developed a highly sensitive and simple method to determine the serum concentrations of choline (PlsCho) and ethanolamine plasmalogen (PlsEtn) separately, using a radioactive iodine and high-performance liquid chromatography (125I-HPLC method) (Maeba & Ueta, 2004; Maeba et al, 2012). The method has improved as auto-analytical system by introducing online detection with flow ┛-counter. We have applied the system to the determination of serum Pls from normal subjects and CAD patients, and investigated the clinical significance of serum Pls as a biomarker for metabolic syndrome and atherosclerosis. This chapter briefly describes Pls structure, distribution, functions, and biosynthesis, and describes in detail the methods for measurement of Pls and clinical significance of serum Pls.
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